Broader environmental adaptation of rare rather than abundant bacteria in reforestation succession soil

Revealing the responses of rare and abundant bacteria to environmental change is crucial for understanding microbial community assembly and ecosystem function. However, both the environmental adaptability and the ecological assembly processes exhibited by rare and abundant soil bacteria remain poorly understood. Here we investigated the assembly processes of rare and abundant bacteria along a chronosequence of a 35-year reforestation succession (8, 17, and 35 years), particularly with regard to their environmental adaptations. Compared to the abundant taxa, the phylogenetic clustering of rare taxa was tighter but their environmental breadth wider. Homogeneous selection (65.8%) belonging to deterministic processes dominated the rare bacterial assembly, whereas homogenizing dispersal and undominated process (57.9%) belonging to stochastic processes governed the abundant taxa. Neutral processes had a significant impact on shaping the rare taxa compared to the abundant taxa. Rare taxa were environmentally less constrained than abundant taxa. Soil EC was the major determinant factor for the assembly processes of both rare and abundant taxa. Ecological assembly processes showed a significant negative correlation with rare bacterial functional redundancies, while they had a significant positive correlation with the abundant taxa. Microbial network modularity further demonstrated that rare taxa developed stronger environmental adaptation strategies than their abundant counterparts. Our study significantly advances the knowledge of the environmental adaptability of rare and abundant bacteria and emphasizes their key role in reforestation ecological succession soils.