适体
傅里叶变换红外光谱
材料科学
介电谱
检出限
量子点
生物传感器
透射电子显微镜
纳米技术
分析化学(期刊)
电化学
化学工程
化学
色谱法
电极
生物
遗传学
工程类
物理化学
作者
Farbod Abazar,Abdollah Noorbakhsh
标识
DOI:10.1016/j.snb.2019.127281
摘要
Considering the importance of measuring insulin in the prevention and diagnosis of diabetes-related illnesses, here we introduced an ultrasensitive electrochemical aptamer-based sensor for detection of insulin in human blood serum. In this research, carbon quantum dots (CQDs) were synthesized using simple, fast and cheap methods from candle soot. By dispersion of the CQDs in chitosan (Chi) matrix, homogenous CQD-Chit nano-composite was obtained. The experimental results demonstrate the good advantages of proposed CQD-Chit nano-composite, such as homogeneity, high stability and multiple amino groups for covalent immobilization of aptamer and fabrication of highly selective insulin aptasensor. CQD-Chit nano-composite was characterized by the transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDAX), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques. Under the optimized conditions with electrochemical impedance spectroscopy technique, a linear response ranges from 0.5 nM to 10 nM with the sensitivity of 80.07 Ω/nM and the very low detection limit of 106.8 pM was obtained for insulin detection. The fabricated aptasensor is validated in comparison with standard methods for detection of insulin in human blood serum. Also, the analytical performances of the fabricated aptasensor can be a superiority compared to the current insulin electrochemical biosensors.
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