核酸外切酶
材料科学
DNA
三元运算
化学
核酸外切酶 III
光电子学
作者
Yanling Hu,Chaoliang Tan,Xin Lin,Zhuangchai Lai,Xiao Zhang,Qipeng Lu,Ning Feng,Dongliang Yang,Lixing Weng
标识
DOI:10.3389/fchem.2019.00844
摘要
Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research.
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