RAD52
生物
同源重组
雷达51
复制蛋白A
解旋酶
DNA修复
DNA
细胞生物学
分子生物学
遗传学
DNA结合蛋白
转录因子
核糖核酸
基因
作者
Zhong Yan,Chuang Xue,Sandeep Kumar,J. Brooks Crickard,Yang Yu,Weibin Wang,Nhung Pham,Yuxi Li,Hengyao Niu,Patrick Sung,Eric C. Greene,Grzegorz Ira
出处
期刊:Molecular Cell
[Elsevier]
日期:2019-12-01
卷期号:76 (5): 699-711.e6
被引量:37
标识
DOI:10.1016/j.molcel.2019.08.017
摘要
Rad52 is a key factor for homologous recombination (HR) in yeast. Rad52 helps assemble Rad51-ssDNA nucleoprotein filaments that catalyze DNA strand exchange, and it mediates single-strand DNA annealing. We find that Rad52 has an even earlier function in HR in restricting DNA double-stranded break ends resection that generates 3' single-stranded DNA (ssDNA) tails. In fission yeast, Exo1 is the primary resection nuclease, with the helicase Rqh1 playing a minor role. We demonstrate that the choice of two extensive resection pathways is regulated by Rad52. In rad52 cells, the resection rate increases from ∼3-5 kb/h up to ∼10-20 kb/h in an Rqh1-dependent manner, while Exo1 becomes dispensable. Budding yeast Rad52 similarly inhibits Sgs1-dependent resection. Single-molecule analysis with purified budding yeast proteins shows that Rad52 competes with Sgs1 for DNA end binding and inhibits Sgs1 translocation along DNA. These results identify a role for Rad52 in limiting ssDNA generated by end resection.
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