生物传感器
介孔材料
微分脉冲伏安法
氧化还原
纳米技术
材料科学
法拉第电流
检出限
电极
线性范围
循环伏安法
吸附
化学
电化学
色谱法
工作电极
无机化学
生物化学
催化作用
有机化学
物理化学
作者
Mostafa Kamal Masud,Jongbeom Na,Tzu En Lin,Victor Malgras,Anant Preet,Abu Ali Ibn Sina,Kathleen Wood,Mutasim Billah,Jeonghun Kim,Jungmok You,Kenya Kani,Andrew E. Whitten,Carlos Salomón,Nam‐Trung Nguyen,Muhammad J. A. Shiddiky,Matt Trau,Md. Shahriar A. Hossain,Yusuke Yamauchi
标识
DOI:10.1016/j.bios.2020.112429
摘要
Advances in nanoarchitectonics enable a wide variety of nanostructured electrodes with tunable shapes and surface for constructing sensitive biosensors. Herein we demonstrate the fabrication of a mesoporous gold (Au) biosensor for the specific and sensitive detection of miRNA in a relatively simple and portable manner. The electrocatalytic activity of the mesoporous Au electrode (MPGE) towards the redox reaction of Fe(CN)6]3-/4- expansively examined. Leveraging the electrocatalytic activity and signal enhancement capacity of the MPGE, an ultrasensitive and specific electrochemical sensor was developed for the detection of microRNA (miRNA). The target miRNA from spiked samples is selectively isolated and purified using magnetic bead-capture probe followed by the direct adsorption on the MPGE through direct affinity interaction between miRNA and mesoporous Au surface. The MPGE-bound miRNA is then quantified by differential pulse voltammetry (DPV) using [Fe(CN)6]4-/3- redox system (Faradaic current decrease with reference to the bare MPGE). This method evades the cumbersome PCR (polymerase chain reaction) and enzymatic amplification steps. This is a single-step assay building which can detect a wide dynamic linear range (100 aM to 1 nM) of miRNA with an ultra-low limit detection of 100 aM and present high translational potentiality for the development of high-performance detection tools for clinics.
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