[Remineralization of demineralized dentin induced by bioactive glass NovaMin].

To explore the remineralization effect of bioactive glass NovaMin on demineralized dentin specimens, and to study the physical and chemical properties of formed structure at dentin surface. Methods: One mm-thickness coronal dentin slices were soaked in ethylene diamine tetraacetic acid (EDTA) for 48 h to prepare the completely demineralized dentin specimens and they were divided into 2 groups: an artificial saliva group (control group) and a NovaMin powder group. The specimens were treated with artificial saliva or NovaMin powder for 2 min (2 times every day), and the interval was 8 hours. Then, the specimens were soaked in the remineralization solution. After 7 days, the scanning electron microscope (SEM), energy dispersive X-ray (EDX), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) were used to detect dentin morphology, the physical and chemical properties of the formed structure at dentin surface. Results: The results of SEM showed that a defined surface layer in the NovaMin powder group could be observed in the SEM imaging at the 7th day, which completely occluded dentinal tubules; the EDX, ATR-FTIR and XRD analysis found that the mineralized layer formed at dentin surface was mainly composed of calcium and phosphate elements, which was similar to the hydroxyapatite-like crystal. However, there were no materials formed at the dentin surface in the control group, and the dentinal tubules were still open. Conclusion: NovaMin can remineralize the demineralized dentin specimens and occlude the dentinal tubules in hydroxyapatite-like crystal structure.目的：探讨生物活性玻璃NovaMin诱导脱矿牙本质再矿化效果，并定性研究再矿化层的理化性质。方法：制备厚度为1 mm冠部牙本质片，用乙二胺四乙酸(ethylenediaminetetraacetic acid，EDTA)浸泡48 h，制备完全脱矿牙本质样本，并平均分为人工唾液组和NovaMin组。每天用人工唾液和生物活性玻璃NovaMin分别处理牙本质片表面2 min，每天2次，间隔8 h，然后将样本浸泡在37 ℃人工唾液中恒温保存。7 d后用扫描电子显微镜(scanning electron microscope，SEM)、能谱分析仪(energy dispersive X-ray，EDX)、傅里叶变换衰减全反射红外光谱(attenuated total reflectance Fourier transform infrared spectroscopy，ATR-FTIR)和X射线衍射仪(X-ray diffraction，XRD)观察和检测牙本质表面矿化物的形成及其理化性质。结果：SEM结果显示：NovaMin组的完全脱矿牙本质表面形成了矿化晶体层，完全封闭了暴露的牙本质小管，经EDX，ATR-FTIR和XRD分析发现这一矿化层主要组分为钙和磷，且结构类似于牙本质磷灰石。而人工唾液组牙本质表面并没有再矿化层的形成，牙本质小管仍然开放。结论：NovaMin能促进完全脱矿的牙本质表面再矿化，形成类似牙本质羟基磷灰石的晶体结构。.