生物
清脆的
计算生物学
基因
基因组编辑
限制
基因组
遗传学
功能(生物学)
Cas9
回文
核糖核酸
DNA
基因组工程
作者
Marie L. Pfeiffer,Joanna Winkler,Daniël Van Damme,Thomas Jacobs,Moritz K. Nowack
标识
DOI:10.1016/j.pbi.2021.102119
摘要
Reverse genetics approaches are routinely used to investigate gene function. However, mutations, especially in critical genes, can lead to pleiotropic effects as severe as lethality, thus limiting functional studies in specific contexts. Approaches that allow for modifications of genes or gene products in a specific spatial or temporal setting can overcome these limitations. The advent of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technologies has not only revolutionized targeted genome modification in plants but also enabled new possibilities for inducible and tissue-specific manipulation of gene functions at the DNA and RNA levels. In addition, novel approaches for the direct manipulation of target proteins have been introduced in plant systems. Here, we review the current development in tissue-specific and conditional manipulation approaches at the DNA, RNA, and protein levels.
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