羧酸酯酶
化学
腺癌
前药
荧光
酶
胰腺癌
生物化学
分子生物学
生物
癌症
遗传学
量子力学
物理
作者
Mengmeng Zhang,Peng Li,Feng Hai,Yan Jia
标识
DOI:10.1016/j.cplett.2021.139143
摘要
• An ICT-based fluorescence probe with large stokes shift was designed for detection of human carboxylesterase 2. • NI-MO has been successfully used as an effective tool in inhibitor screening. • Probe NI-MO with lysosomal carboxylesterase 2 labeling ability and can be used for cell imaging. • The potential biological applications of the probe, which could be applied to Identify pancreatic cancer cells. Carboxylesterase 2 as a critical hydrolase, current studies are limited to its role in prodrug metabolism while functional studies found that pancreatic adenocarcinoma (PAAD) is associated with expression differences of this enzyme. However, it lacks of organelle level assessment of carboxylesterase 2 activity in the fluorescent tools. Herein, we develop a fluorescent probe (NI-MO) that achieves selective visualization of human lysosomal carboxylesterase 2 in living cells to distinguish PAAD and normal cells, which provide a new avenue to guide pancreatic adenocarcinoma profiling. Notably, we constructed a high-throughput screening approach to rapidly screen the potential inhibitors through carboxylesterase 2 activity.
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