玉米赤霉烯酮
检出限
量子点
碲化镉光电
化学
色谱法
荧光
水溶液
分析化学(期刊)
纳米技术
材料科学
真菌毒素
物理
食品科学
光学
物理化学
作者
Fuyuan Zhang,Bing Liu,Wei Sheng,Yan Zhang,Qi Liu,Shijie Li,Shuo Wang
标识
DOI:10.1016/j.foodchem.2018.02.060
摘要
CdTe/CdS/ZnS quantum dots (QDs) were synthesized in aqueous phase and conjugated with a new anti-zearalenone (ZEN) monoclonal antibody. Using this novel fluorescent probe, a fluoroimmunoassay (FLISA) and a rapid immunochromatographic strip (ICTS) were developed for the detection of ZEN in maize. Our proposed FLISA allowed for ZEN determination in the range of 0.038–0.977 ng/mL with an IC50 of 0.162 ng/mL and a limit of detection (LOD) of 0.012 ng/mL occurring in maize. The rapid ICTS had a visual LOD of 1.0 ng/mL in buffer solution and 1.5 ng/mL in maize extract. These two QDs-based immunoassays were all successfully verified by commercial ELISA kits. The results confirmed that: firstly, the FLISA can be used as a robust method for the sensitive detection of ZEN; and secondly, the ICTS is ideally suited for rapidly screening large numbers of samples.
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