光致变色
荧光
异构化
荧光蛋白
化学
生物系统
对比度(视觉)
量子
材料科学
计算机科学
光化学
光学
有机化学
物理
绿色荧光蛋白
人工智能
生物化学
生物
基因
量子力学
催化作用
作者
Anaïs C. Bourges,Benjamien Moeyaert,Thi Yen Hang Bui,Franziska Bierbuesse,Wim Vandenberg,Peter Dedecker
摘要
In this study, we develop a general analytical model of the photochromism of fluorescent proteins and apply it to spectroscopic measurements performed on six different labels. Our approach provides quantitative explanations for phenomena such as the existence of positive and negative switching, limitations in the photochromism contrast, and the fact that initial switching cycles may differ from subsequent ones. It also allows us to perform the very first measurement of all four isomerization quantum yields involved in the switching process.
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