细胞外小泡
2019年冠状病毒病(COVID-19)
药物输送
药品
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
2019-20冠状病毒爆发
靶向给药
细胞外
化学
药理学
病毒学
细胞生物学
生物
医学
生物化学
传染病(医学专业)
病理
疾病
有机化学
爆发
作者
Yao Zhang,Sheng-Jiao Song,Jin He,Zhuo-Hua Zhao,Ke Zhuang,Yuan Zhang,Xing Li
出处
期刊:Current Pharmaceutical Biotechnology
[Bentham Science]
日期:2024-03-28
卷期号:25
标识
DOI:10.2174/0113892010282251240324123038
摘要
Background:: Extracellular vesicles (EVs) are emerging as potential drug carriers in the fight against COVID-19. This study investigates the ability of EVs as drug carriers to target SARS-CoV-2-infected cells. Methods:: EVs were modified using Xstamp technology to carry the virus’s RBD, enhancing targeting ability to hACE2+ cells and improving drug delivery efficiency. Characterization confirmed EVs’ suitability as drug carriers. For in vitro tests, A549, Caco-2, and 4T1 cells were used to assess the targeting specificity of EVRs (EVs with membrane-surface enriched RBD). Moreover, we utilized an ex vivo lung tissue model overexpressing hACE2 as an ex vivo model to confirm the targeting capability of EVRs toward lung tissue. The study also evaluated drug loading efficiency and assessed the potential of the anti-inflammatory activity on A549 lung cancer cells exposed to lipopolysaccharide. Results: demonstrate the successful construction of RBD-fused EVRs on the membrane-surface. In both in vitro and ex vivo models, EVRs significantly enhance their targeting ability towards hACE2+ cells, rendering them a safe and efficient drug carrier. Furthermore, ultrasound loading efficiently incorporates IL-10 into EVRs, establishing an effective drug delivery system that ameliorates the pro-inflammatory response induced by LPS-stimulated A549 cells. Conclusion:: These findings indicate promising opportunities for engineered EVs as a novel nanomedicine carrier, offering valuable insights for therapeutic strategies against COVID-19 and other diseases.
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