硫酸化
多糖
化学
单糖
化学结构
区域选择性
反应性(心理学)
X射线光电子能谱
核磁共振波谱
有机化学
生物化学
催化作用
化学工程
医学
替代医学
病理
工程类
作者
Junlong Wang,Wen Yang,Jiancheng Wang,Xia Wang,Fang Wu,Jian Yao,Ji Zhang,Ziqiang Lei
标识
DOI:10.1016/j.carbpol.2015.07.030
摘要
The biological activities of sulfated polysaccharides are related to the substitution positions of functional groups. In this study, regioselective sulfation of Artemisia sphaerocephala polysaccharides (SRSASP) was prepared by using triphenylchloromethane (TrCl) as protecting precursor. FT-IR spectra and X-ray photoelectron spectroscopy (XPS) showed that SO(3-) group (S(6+), high binding energy of 168.7eV) was widely present in sulfated polysaccharides. (13)C NMR spectroscopy showed that C-2 and C-3 substitution was occurred but not fully sulfation. Meanwhile, C-6 substituted signals near 65ppm were not observed. The degree of substitution varied from 0.44 to 0.63 in SRSASP which could be attributed to the low reactivity at secondary hydroxyl. Monosaccharide composition result showed a decrease in the ratio of mannose/glucose, indicating the change of chemical composition in sulfated polysaccharides. In size-exclusion chromatograph analysis, a decrease in molecular weight and broadening of molecular weight distribution of sulfated polysaccharides was also observed. It could be attributed to the hydrolysis of polysaccharide in the sulfated reaction.
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