木槿
花青素
木槿
分离(微生物学)
生药学
传统医学
化学
植物
食品科学
生物活性
生物
医学
生物化学
体外
微生物学
作者
Claudia Grajeda‐Iglesias,Maria‐Cruz Figueroa‐Espinoza,Nathalie Barouh,Bruno Baréa,Ana Fernandes,Víctor de Freitas,Erika Salas
标识
DOI:10.1021/acs.jnatprod.5b00958
摘要
The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid–base equilibrium (pK′a 2.92), the acidity (pKa 3.70), and the hydration constants (pKh 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract.
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