CD86
CD80
单克隆抗体
分泌物
抗原
CD8型
T细胞
抗体
分子生物学
免疫学
生物
细胞生物学
细胞毒性T细胞
化学
CD40
免疫系统
体外
内分泌学
生物化学
作者
Atsushi Nakazawa,Iris Dotan,Jens Brimnes,Matthieu Allez,Ling Shao,Fumihiko Tsushima,Miyuki Azuma,Lloyd Mayer
标识
DOI:10.1053/j.gastro.2004.02.004
摘要
Previous studies have suggested that intestinal epithelial cells (IECs) may function as antigen-presenting cells for CD4+ and CD8+ T cells. However, these cells fail to express conventional costimulatory molecules (CD80, CD86), leading to the possibility that antigen presented by normal IECs could result in anergy. Other members of the B7 family have recently been identified. B7h interacts with inducible costimulator (ICOS) on T cells and provides a positive signal, whereas B7-H1 and B7-DC interact with PD-1 and transmit an inhibitory signal. Our aim was to determine whether IECs express novel B7 family members and whether these molecules play a role in IEC:T-cell interactions.B7h and B7-H1 expression was assessed in isolated IECs and IEC lines. The functional role of B7h and B7-H1 in the interaction between IECs and T cells was assessed in coculture experiments using purified anti-B7h or B7-H1 monoclonal antibodies (mAbs), B7h immunoglobulin (Ig), or B7-H1 fusion proteins.B7h and B7-H1 messenger RNA was detected in IEC lines and IECs from healthy controls and patients with inflammatory bowel disease (IBD). IECs from patients with IBD but not healthy controls expressed B7h and B7-H1 protein on their surface. Proliferation of IEC-stimulated T cells was inhibited only by B7h immunoglobulin treatment, whereas interferon gamma secretion in these cocultures was inhibited by both anti-B7h mAb and B7h Ig. No difference was seen between IBD or normal IEC populations.These data suggest that the B7h-ICOS costimulatory pathway may be important in IEC:T-cell interactions.
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