巨噬细胞极化
自噬
巨噬细胞
泡沫电池
化学
细胞生物学
福克斯O1
生物
体外
信号转导
生物化学
蛋白激酶B
细胞凋亡
作者
Xueying Li,Yujing Wang,Shun Chen,Li‐Hua Pan,Qiang-Ming Li,Jian‐Ping Luo,Xue‐Qiang Zha
标识
DOI:10.1021/acs.jafc.1c07483
摘要
The present work aimed to explore the effect and underlying mechanism of a homogeneous Laminaria japonica polysaccharide (LJP61A) on macrophage polarization in high-fat-diet-fed LDLr–/– mice and Ox-LDL-induced macrophages. Results showed that LJP61A remarkably reduced the lesion burden in atherosclerotic mice, alleviated lipid deposition in Ox-LDL-stimulated macrophages, decreased the expression of M1 macrophage markers, and increased the expression of M2 macrophage markers, thus reducing the M1/M2 macrophage phenotype ratio. Meanwhile, the autophagic flux of macrophages was enhanced by LJP61A treatment in vitro and in vivo. 3-Methyladenine is an autophagic inhibitor. As expected, this inhibitor blocked the effects of LJP61A on macrophage polarization. SIRT1 and FoxO1 are two key upstream genes that control the autophagy behavior. We also found that LJP61A significantly up-regulated the expression of SIRT1 and FoxO1. However, these effects of LJP61A were abolished by the SIRT1 siRNA and FoxO1 inhibitor AS1842856. These results suggested that LJP61A reduced atherosclerosis in HFD-induced LDLr–/– mice via regulating autophagy-mediated macrophage polarization.
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