甜菜碱
活性氧
斑马鱼
过氧化氢酶
超氧化物歧化酶
氧化应激
化学
生物化学
抗氧化剂
谷胱甘肽
超氧化物
细胞生物学
生物
分子生物学
酶
基因
作者
Ao Li,Yaqi Gu,Xiuzhen Zhang,Huan Yu,Dongwu Liu,Qiuxiang Pang
标识
DOI:10.3389/fphys.2022.877178
摘要
When fish are under oxidative stress, levels of reactive oxygen species (ROS) are chronically elevated, which play a crucial role in fish innate immunity. In the present study, the mechanism by which betaine regulates ROS production via Wnt10b/β-catenin signaling was investigated in zebrafish liver. Our results showed that betaine enrichment of diet at 0.1, 0.2 and 0.4 g/kg induced Wnt10b and β-catenin gene expression, but suppressed GSK-3β expression in zebrafish liver. In addition, the content of superoxide anion (O 2 ·− ), hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (·OH) was decreased by all of the experimental betaine treatments. However, betaine enrichment of diet at 0.1, 0.2 and 0.4 g/kg enhanced gene expression and activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) in zebrafish liver. In addition, Wnt10b RNA was further interfered in zebrafish, and the results of Wnt10b RNAi indicated that Wnt10b plays a key role in regulating ROS production and antioxidant enzyme activity. In conclusion, betaine can inhibit ROS production in zebrafish liver through the Wnt10b/β-catenin signaling pathway.
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