化学
配体(生物化学)
血红素
活动站点
组合化学
立体化学
异核分子
辅因子
血红素蛋白
胺气处理
金属
硫酯
肽
生物化学
有机化学
催化作用
核磁共振波谱
酶
受体
作者
Chen Yong-lu,Yi Zhou,Chunmao He
标识
DOI:10.1016/j.cclet.2023.108251
摘要
Herein, we report a semi-synthetic strategy affording a nitrophorin 2 (NP2) variant with a N,N′-bis(2-pyridylmethyl)amine (Dpa) ligand as sidechain selectively installed at position 27, which was assembled from a synthetic peptide thioester bearing the Dpa ligand and an expressed protein segment via native chemical ligation. The semi-synthetic NP2 was able to accept the natural heme b cofactor and the Dpa ligand was able to bind Cu(II)/Fe(III) ions, leading to heteronuclear active sites
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