Anti-allergic and anti-Inflammatory Effects of Cornus officinalis using Zebrafish and IgE-chip Technology

Abstract ID 53659 Poster Board 354 Atopic dermatitis (AD) is a inflammatory skin disease and itch is the most important symptom of AD. Immunoglobulin E (IgE) plays a critical role in allergic reactions. The IgE-chip technology involves the identification of allergenic proteins in natural ingredient. To find the natural products on anti-allergic effect, 229 of natural extracts were screened using IgE/FcεR1-chip assay and we selected Cornus officinalis extract (CO, code no. 13-12-0092). CO reduced the Compound48/80-induced histamine production in a dose dependent manner (25, 50, 100 ug/mL) and reactive oxygen species generation in MC/9 mast cells. We tested migration of neutrophils from tail fin wounds by CO in a transgenic zebrafish Tg(mpx:GFP) expressing GFP. In conculsion, the IgE/FcεR1-chip technology is a rapid method to find the effective ingredient that do not cause allergic reaction and CO is a potential anti-inflammatory mediator for treatment of allergic and inflammatory skin disease. This article was supported by the Korea Institute of Oriental Medicine (KSN20223302) and the Technology Development Program (S3099171) funded by the Ministry of SMEs and Sartups (Mss, Korea).