多糖
色谱法
萃取(化学)
化学
凝胶渗透色谱法
琼脂糖
柱色谱法
聚合物
生物化学
有机化学
酶
作者
Xudan Guo,Haoran Shen,Fan Gao,Xueting Wang,Xinyuan Deng,Jiahui Zhai,Jing Zhang,Xiaolong Ji
标识
DOI:10.1002/star.202300120
摘要
Abstract The objective of this paper is to analyze and contrast the features of the structure of jujube polysaccharides extracted and purified using various techniques, four fractions, namely hot water extraction and 0.3 M NaCl‐PBS purification (PZMP3‐1 and PZMP3‐2), alkaline extraction and 0.3 M NaCl purification (SAZMP4), alkaline extraction and 0.3 M‐PBS NaCl purification (PZJRP), are obtained by column chromatography (Sephacryl S‐300) and Fast Flow (DEAE‐Sepharose). Chemical testing, gas chromatography, scanning electron microscopy, nuclear magnetic resonance spectroscopy, methylation analysis, high‐performance gel permeation chromatography, and atomic force microscopy are used to examine and evaluate their properties. Different purification methods have certain effects on the structural characteristics of a series of jujube polysaccharides. The results can provide fundamental knowledge to select desirable extraction and purification technologies for the preparation of pure jujube polysaccharides.
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