氧化还原
电化学
纳米片
Nafion公司
生物传感器
电极
电催化剂
电子转移
化学
石墨氮化碳
肌红蛋白
无机化学
离子液体
材料科学
化学工程
纳米技术
催化作用
有机化学
光催化
物理化学
工程类
作者
Ying Ding,Wen Zhang,Guoan Luo,Hongmei Xie,Juan Liu,Yaru Xi,Guangjiu Li,Wei Sun
出处
期刊:Current Analytical Chemistry
[Bentham Science]
日期:2020-08-13
卷期号:16 (6): 703-710
被引量:3
标识
DOI:10.2174/1573411015666190710223818
摘要
Background: Carbon-based nanomaterials, especially carbon nitride (C3N4) has attracted tremendous interest in biosensor applications. Meanwhile, the mechanism of redox protein sensing and related electrocatalytic reactions can provide a valid basis for understanding the process of biological redox reaction. Objective: The aim of this paper is to construct a new electrochemical enzyme sensor to achieve direct electron transfer of myoglobin (Mb) on CILE surface and display electrocatalytic reduction activity to catalyze trichloroacetic acid (TCA) and H2O2. Methods: The working electrode was fabricated based on ionic liquid modified Carbon Paste Electrode (CILE) and C3N4 nanosheets were modified on the CILE surface, then Mb solution was fixed on C3N4/CILE surface and immobilized by using Nafion film. The as-prepared biosensor displayed satisfactory electrocatalytic ability towards the reduction of TCA and H2O2 in an optimum pH 7.0 buffer solution. Results: The results indicated that C3N4 modified electrode retained the activity of the enzyme and displayed quasi-reversible redox behavior in an optimum pH 7.0 buffer solution. The electrochemical parameters of the immobilized Mb on the electrode surface were further calculated with the results of the electron transfer number (n) as 1.27, the charge transfer coefficient (α) as 0.53 and the electrontransfer rate constant (ks) as 3.32 s-1, respectively. The Nafion/Mb/C3N4/CILE displayed outstanding electrocatalytic reduction activity to catalyze trichloroacetic acid and H2O2. Conclusion: The Nafion/Mb/C3N4/CILE displayed outstanding electrocatalytic reduction, which demonstrated the promising applications of C3N4 nanosheet in the field electrochemical biosensing.
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