Isolation, Identification and Genomic Analysis of Orange-Spotted Grouper Iridovirus Hainan Strain in China

石斑鱼 虹彩病毒 生物 开放式参考框架 病毒学 水产养殖 石斑鱼 GenBank公司 病毒 渔业 遗传学 基因 打开阅读框 肽序列
作者
Helong Cao,Dongzhuo Zhang,Guanghui Mu,Siting Wu,Yurong Tu,Qiwei Qin,Jingguang Wei
出处
期刊:Viruses [Multidisciplinary Digital Publishing Institute]
卷期号:16 (10): 1513-1513
标识
DOI:10.3390/v16101513
摘要

The orange-spotted grouper (Epinephelus coioides) is an important mariculture fish in China. However, in recent years, with the rapid development of aquaculture activities, outbreaks of viral diseases have affected the grouper aquaculture industry, causing severe economic losses. In the present study, we isolated and identified a virus from diseased, orange-spotted groupers from an aquaculture farm in Hainan Province, China. The isolated virus was identified as orange-spotted grouper iridovirus, hence named the orange-spotted grouper iridovirus Hainan strain (OSGIV-HN-2018-001). OSGIV-HN-2018-001 induces a cytopathic effect after the infection of mandarin fish (Siniperca chuatsi) brain clonal passage (SBC) cells. In addition, the cytoplasm of the OSGIV-HN-2018-001-infected SBC cells was found to contain a large number of hexagonal virus particles with a diameter of approximately 134 nm. Using the Illumina NovaSeq system, we assembled the sequence data and annotated the complete genome of OSGIV-HN-2018-001 (GenBank accession number: PP974677), which consisted of 110,699 bp and contained 122 open reading frames (ORFs). Phylogenetic tree analysis showed that OSGIV-HN-2018-001 was most closely related to ISKNV-ASB-23. The cumulative mortality rate of groupers infected with OSGIV-HN-2018-001 reached 100% on day 8. The spleens were enlarged and blackened after the dissection of the dying groupers. These results contribute to the understanding of the molecular regulatory mechanism of the iridovirus infection and provide a basis for iridovirus prevention.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
阿里嘎多完成签到,获得积分10
1秒前
Ava应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
JamesPei应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
Hello应助科研通管家采纳,获得10
2秒前
小蘑菇应助科研通管家采纳,获得10
3秒前
鑫叶应助科研通管家采纳,获得20
3秒前
Hello应助科研通管家采纳,获得10
3秒前
Jasper应助科研通管家采纳,获得10
3秒前
丘比特应助科研通管家采纳,获得10
3秒前
3秒前
今后应助科研通管家采纳,获得10
3秒前
3秒前
领导范儿应助科研通管家采纳,获得10
3秒前
科研通AI5应助科研通管家采纳,获得10
3秒前
852应助武雨寒采纳,获得10
3秒前
科研通AI2S应助科研通管家采纳,获得10
3秒前
酷波er应助科研通管家采纳,获得10
3秒前
赘婿应助科研通管家采纳,获得10
3秒前
英俊的铭应助科研通管家采纳,获得10
4秒前
小宝发布了新的文献求助10
4秒前
4秒前
6秒前
阿里嘎多发布了新的文献求助10
7秒前
沉静青旋完成签到,获得积分10
7秒前
7秒前
7秒前
McGrady发布了新的文献求助10
8秒前
LLL发布了新的文献求助10
9秒前
Szj完成签到,获得积分10
11秒前
orixero应助登山人采纳,获得10
11秒前
沉静青旋发布了新的文献求助10
12秒前
13秒前
14秒前
14秒前
14秒前
天明发布了新的文献求助10
14秒前
高分求助中
Technologies supporting mass customization of apparel: A pilot project 600
Introduction to Strong Mixing Conditions Volumes 1-3 500
Tip60 complex regulates eggshell formation and oviposition in the white-backed planthopper, providing effective targets for pest control 400
A Field Guide to the Amphibians and Reptiles of Madagascar - Frank Glaw and Miguel Vences - 3rd Edition 400
Optical and electric properties of monocrystalline synthetic diamond irradiated by neutrons 320
共融服務學習指南 300
Essentials of Pharmacoeconomics: Health Economics and Outcomes Research 3rd Edition. by Karen Rascati 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3800426
求助须知:如何正确求助?哪些是违规求助? 3345655
关于积分的说明 10326568
捐赠科研通 3062128
什么是DOI,文献DOI怎么找? 1680879
邀请新用户注册赠送积分活动 807263
科研通“疑难数据库(出版商)”最低求助积分说明 763572