An LC‐MS/MS Method Development and Validation for the Quantification of Abametapir in Plasma Samples

ABSTRACT The major goal of the current research study was to establish a sensitive tandem mass spectrometric method using electrospray ionization and liquid chromatography for quantifying abametapir in biological matrices. An Inertsil ODS chromatographic column with dimensions of 50 × 4.60 mm and 5.0 µm particles size was used to achieve chromatographic elution. Injection volume was set to 10.0 µL. Flow rate and stationary phase oven temperature were retained at 0.70 mL/min and 25.0°C. Isocratic elution was executed with acetonitrile, 0.10% v/v HCOOH, and methanol in a fraction of 20:10:70 (v/v/v) as the mobile phase. On multiple reaction monitoring, precursor‐product ion transitions were monitored at m/z 185.1→106.06 for abametapir and 287.16→269.15 for the abacavir internal standard. The drug's linearity graph had an r 2 value of 0.9998 and was rectilinear at concentrations between 2.6 and 104 ng/mL. The inter‐ and intrabatch precision % relative standard deviation values ranged from 2.57 to 4.53. In short, the method that was made has been proven to work and validated as per the regulatory guidelines.