Man's activities have had profound, and usually negative, influences on freshwater fishes from the smallest streams to the largest rivers. Some negative effects are due to contaminants, while others are associated with changes in watershed hydrology, habitat modifications, and alteration of energy sources upon which the aquatic biota depends. Regrettably, past efforts to evaluate effects of man's activities on fishes have attempted to use water quality as a surrogate for more comprehensive biotic assessment. A more refined biotic assessment program is required for effective protection of freshwater fish resources. An assessment system proposed here uses a series of fish community attributes related to species composition and ecological structure to evaluate the quality of an aquatic biota. In preliminary trials this system accurately reflected the status of fish communities and the environment supporting them.