同四聚体
离子强度
化学
色谱法
聚丙烯酰胺凝胶电泳
细胞内
葡聚糖
生物化学
生物物理学
酶
水溶液
有机化学
生物
蛋白质亚单位
基因
作者
Yunsheng Zhao,Xiaolan Yang,Xiaoyan Li,Youquan Bu,Peihong Deng,Chun Zhang,Jia‐Xun Feng,Yanling Xie,Sha Zhu,Huidong Yuan,Miao Yu,Fei Liao
摘要
An intracellular uricase from Bacillus fastidiosus with high catalytic capacity and strong resistance to xanthine was inactivated in water but could be essentially re-activated in solutions of high ionic strength. By polyacrylamide gel electrophoresis (PAGE), gradient PAGE, sodium-dodecyl-sulfate-PAGE, gel-filtration through Sephadex G200, and activity staining with peroxidase and its chromatogenic substrate, this homotetrameric uricase in water was found to dissociate into inactive homodimers that could form active homotetramers again in solutions of high ionic strength. Sensitivity to low ionic strength of solutions complicates formulation of this uricase as a drug and its elimination requires protein engineering.
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