化学
尿酸
检出限
生物化学
尿酸氧化酶
色谱法
生物传感器
线性范围
酶
荧光
核化学
量子力学
物理
作者
Yuan Jiao,Yunlong Xing,Kai Li,Zainan Li,Guoqing Zhao
摘要
In this study, an enzyme-coupled fluorescence sensor was developed successfully for the sensitive detection of uric acid (UA) and uricase inhibitor based on graphene quantum dots (GQDs). UA was first oxidized using uricase and produced hydrogen peroxide (H2 O2 ), which could oxidize phenol to benzoquinone in the presence of horseradish peroxidase (HRP) as the catalysis. Benzoquinone is an efficient quencher and can cause fluorescence quenching of GQDs. The degree of quenching was proportional to UA concentration and was linear for the UA concentration in the ranges 0.2-14 μmol/L. The detection limit was 0.06 μmol/L for UA. In addition, the proposed sensor was further utilized for UA detection in human serum samples with satisfactory reproducibility and accuracy. The proposed strategy provided may be widely utilized to sense H2 O2 or H2 O2 generating processes in related biological environment.
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