单细胞分析
细胞内
免疫分析
微流控
细胞
化学
蛋白质组学
生物物理学
定量蛋白质组学
纳米技术
材料科学
生物化学
生物
抗体
基因
免疫学
作者
Weizhi Liu,Ruihua Zhang,Shanqing Huang,Xingrui Li,Wanling Liu,Jian Zhou,Lin Zhu,Yanling Song,Chaoyong Yang
出处
期刊:Langmuir
[American Chemical Society]
日期:2022-06-24
卷期号:38 (26): 7929-7937
被引量:3
标识
DOI:10.1021/acs.langmuir.2c00341
摘要
Unlike conventional bulk measurements, single-cell protein analysis permits quantification of protein expression in individual cells. This has shed light on the cell-to-cell variation in heterogeneous biological systems, such as solid tumors, brain tissues, and developing embryos. Herein, a microfluidic method is developed to profile protein expression in individual cells by performing single-cell intracellular protein immunoassay in picoliter paired droplets. The high sensitivity of single-cell protein analysis on a chip is achieved by the confined reaction volume of picoliter droplets, efficient kinetic characteristics of the immunoassay through active mixing, and minimum single-cell protein loss by integrated operations. The abundance of an intracellular prostate specific antigen at the single-cell level is measured, and then the platform is applied to identify cell types and investigate heterogeneity within cell populations. Overall, a paired chip for single-cell immunoassay establishes a foundation for parallel, sensitive, and integrated protein quantification at the single-cell level and will find wide applications in the field of single-cell proteomics.
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