Highly sensitive SERS substrates with multi-hot spots for on-site detection of pesticide residues

Pesticide residues will be a huge threat to food security and ecological environment; therefore, there is an urgent need to achieve rapid and on-site detection of pesticide residues. Herein, a plasmonic substrate with multiple "hot spots" was fabricated by transferring three-dimensional (3D) Au nanoparticles (NPs) onto the polydimethylsiloxane (PDMS) membrane for highly sensitive surface-enhanced Raman scattering (SERS) detection of pesticide residues. In combination with 3D-FDTD simulations, high SERS enhancement (EF = 1.2 × 108) and high detection sensitivity (LOD = 6.3 × 10-10 M) were achieved, mainly due to the enhanced electromagnetic fields around the "hot spots". Additionally, the PDMS-based SERS substrate held good transparency and flexibility, enabling conformal contact with non-planar surfaces and allowing the laser to penetrate the back of the analytes. Combined with a portable Raman spectrometer, the substrates holds great potential for rapid, high-sensitive, and on-site detection of contaminants in food, especially for the analyte on the nonplanar surfaces.