Urine antimony and risk of cardiovascular disease – A prospective case-cohort study in Danish Non-Smokers

Limited evidence suggests that antimony induces vascular inflammation and oxidative stress and may play a role in cardiovascular disease (CVD) risk. However, few studies have examined whether environmental antimony from sources other than tobacco smoking is related with CVD risk. The general population may be exposed through air, drinking water, and food that contains antimony from natural and anthropogenic sources, such as mining, coal combustion, and manufacturing. To examine the association of urinary antimony with incident acute myocardial infarction (AMI), heart failure, and stroke among people who never smoked tobacco. Between 1993-1997, the Danish Diet, Cancer and Health (DCH) cohort enrolled participants (ages 50-64 years), including n=19,394 participants who reported never smoking at baseline. Among these never smokers, we identified incident cases of AMI (N= 809), heart failure (N= 958), and stroke (N=534) using the Danish National Patient Registry. We also randomly selected a subcohort of 600 men and 600 women. We quantified urine antimony concentrations in samples provided at enrollment. We used modified Cox proportional hazards models to estimate adjusted hazard ratios (HR) for each incident CVD outcome in relation to urine antimony, statistically adjusted for creatinine. We used a separate prospective cohort, the San Luis Valley Diabetes Study (SLVDS), to replicate these results. In the DCH cohort, urine antimony concentrations were positively associated with rates of AMI and heart failure (HR= 1.52; 95%CI= 1.12, 2.08 and HR=1.58; 95% CI= 1.15, 2.18, respectively, comparing participants in the highest (>0.09µg/L) with the lowest quartile (<0.02 µg/L) of antimony). In the SLVDS cohort, urinary antimony was positively associated with AMI, but not heart failure. Among this sample of Danish people who never smoked, we found that low levels of urine antimony are associated with incident CVD. These results were partially confirmed in a smaller US cohort.