丙二醛
脂质过氧化
毛细管电泳
化学
硫代巴比妥酸
色谱法
衍生化
活性氧
生物化学
氧化应激
高效液相色谱法
作者
Maria Patrícia do Nascimento,Bruna Marchiori Berlande,Marina Guedes Fraga Lopes,Mário Flávio Cardoso de Lima,Cláudio Teodoro de Souza,Marcone Augusto Leal de Oliveira
标识
DOI:10.1080/10408347.2023.2296948
摘要
Lipid peroxidation occurs when substances, such as reactive oxygen species, attack lipids. Polyunsaturated fatty acids are the main targets. Several products are formed, including primary products such as lipid hydroperoxides and secondary products such as malondialdehyde (MDA), the most used lipid peroxidation biomarker. As MDA levels are elevated in several diseases, MDA is an essential indicator for assessing pathological states. The thiobarbituric acid reactive substances assay is the most widely used method for MDA determination. However, it lacks specificity. Capillary Electrophoresis (CE) is a separation technique that has been used to quantify MDA in biological samples. This technique has advantages such as the low amount of biological sample required, absence or low volume of organic solvent, short analysis time, separation of interferents, sample preparation step with only protein precipitation, and the possibility of direct detection of the MDA, without derivatization. To our knowledge, this review article is the first to show the CE background for analyzing MDA in biological samples. Therefore, given the potential of MDA in evaluating pathological states, this article demonstrates the potential of CE to become a reference method for MDA determination in clinical analysis laboratories, which will play a significant role in diagnosing and monitoring diseases.
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