Brilliant Pitaya‐Type Silica Colloids with Central–Radial and High‐Density Quantum Dots Incorporation for Ultrasensitive Fluorescence Immunoassays

Abstract Creating secondary nanostructures from fundamental building blocks with simultaneous high loading capacity and well‐controlled size/uniformity, is highly desired for nanoscale synergism and integration of functional units. Here a novel strategy is reported for hydrophobic quantum dots (QDs) assembley with porous templates, to form pitaya‐type fluorescent silica colloids with densely packed and intact QDs throughout the silica matrix. The mercapto‐terminated dendritic silica spheres with highly accessible central–radial pores and metal‐affinity interior surface, are adopted as a powerful absorbent host for direct immobilization of QDs from organic phase with high loading capacity. The alkylsilane mediated silica encapsulation prevents QDs' optical degradation induced by ligand exchange and favors the homogeneous silica shell formation. These multiple QD embedded silica spheres exhibit good compatibility for different colored QDs with well‐preserved fluorescence, high colloidal/optical stability, and versatile surface functionality. It is demonstrated that after integration with a lateral flow strip platform, these silica colloids provide an ultrasensitive, specific, and robust immunoassay for C‐reaction protein in clinical samples as promising fluorescent reporters.