葡萄糖氧化酶
生物传感器
化学
动力学
固定化酶
生物分子
离子强度
酶动力学
膜
酶催化
反应性(心理学)
化学工程
米氏-门汀动力学
纳米技术
酶
材料科学
有机化学
酶分析
活动站点
生物化学
水溶液
医学
物理
替代医学
病理
量子力学
工程类
作者
Jiachao Yu,Yuanjian Zhang,Songqin Liu
标识
DOI:10.1016/j.bios.2013.12.042
摘要
The construction of nanodevices coupled with an integrated real-time detection system for evaluation of the function of biomolecules in biological processes, and enzymatic reaction kinetics occurring at the confined space or interface is a significant challenge. In this work, a nanochannel-enzyme system in which the enzymatic reaction could be investigated with an electrochemical method was constructed. The model system was established by covalently linking glucose oxidase (GOD) onto the inner wall of the nanochannels of the porous anodic alumina (PAA) membrane. An Au disc was attached at the end of the nanochannels of the PAA membrane as the working electrode for detection of H2O2 product of enzymatic reaction. The effects of ionic strength, amount of immobilized enzyme and pore diameter of the nanochannels on the enzymatic reaction kinetics were illustrated. The GOD confined in nanochannels showed high stability and reactivity. Upon addition of glucose to the nanochannel-enzyme system, the current response had a calibration range span from 0.005 to 2 mM of glucose concentration. The apparent Michaelis-Menten constant (K(m)(app)) of GOD confined in nanochannel was 0.4 mM. The presented work provided a platform for real-time monitoring of the enzyme reaction kinetics confined in nanospaces. Such a nanochannel-enzyme system could also help design future biosensors and enzyme reactors with high sensitivity and efficiency.
科研通智能强力驱动
Strongly Powered by AbleSci AI