蛹虫草
蘑菇
分离(微生物学)
冬虫夏草
生物
多糖
毛虫
药用真菌
植物
微生物学
幼虫
生物化学
作者
Liehuang Zhu,Qingjiu Tang,Shuai Zhou,Yan Fang Liu,Zhang Zhong,Gao Xin-hua,Shiping Wang,Zhaolong Wang
出处
期刊:International Journal of Medicinal Mushrooms
日期:2014-01-01
卷期号:16 (3): 247-257
被引量:21
标识
DOI:10.1615/intjmedmushr.v16.i3.50
摘要
A novel polysaccharide (CP2-S) was purified from Cordyceps militaris fruit bodies by hot water extraction, ethanol precipitation, DEAE-Sepharose Fast Flow and Sephacryl S-400 high-resolution chromatography. The polysaccharide had a molecular weight of 5.938 × 106 g/mol and was mainly composed of glucose. CP2-S had carbohydrate content estimated to be 100% using the phenol-sulfuric acid method. Immunostimulating experiments in vitro indicated that CP2-S could stimulate nitric oxide production, phagocytosis, respiratory burst activity, and secretion of interleukin-1β and interleukin-2 of macrophages, suggesting that this water-soluble polysaccharide from the fruit body of C. militaris is a natural immunostimulating polysaccharide with potential for further application.
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