小RNA
医学
细胞凋亡
再灌注损伤
细胞生物学
程序性细胞死亡
心力衰竭
生物
坏死性下垂
癌症研究
内科学
心肌细胞
下调和上调
纤维化
心脏纤维化
作者
Arin B. Aurora,Ahmed I. Mahmoud,Xiang Luo,Brett A. Johnson,Eva van Rooij,Satoshi Matsuzaki,Kenneth M. Humphries,Joseph A. Hill,Rhonda Bassel-Duby,Hesham A. Sadek,Eric N. Olson
摘要
Early reperfusion of ischemic cardiac tissue remains the most effective intervention for improving clinical outcome following myocardial infarction. However, abnormal increases in intracellular Ca2+ during myocardial reperfusion can cause cardiomyocyte death and consequent loss of cardiac function, referred to as ischemia/reperfusion (IR) injury. Therapeutic modulation of Ca2+ handling provides some cardioprotection against the paradoxical effects of restoring blood flow to the heart, highlighting the significance of Ca2+ overload to IR injury. Cardiac IR is also accompanied by dynamic changes in the expression of microRNAs (miRNAs); for example, miR-214 is upregulated during ischemic injury and heart failure, but its potential role in these processes is unknown. Here, we show that genetic deletion of miR-214 in mice causes loss of cardiac contractility, increased apoptosis, and excessive fibrosis in response to IR injury. The cardioprotective roles of miR-214 during IR injury were attributed to repression of the mRNA encoding sodium/calcium exchanger 1 (Ncx1), a key regulator of Ca2+ influx; and to repression of several downstream effectors of Ca2+ signaling that mediate cell death. These findings reveal a pivotal role for miR-214 as a regulator of cardiomyocyte Ca2+ homeostasis and survival during cardiac injury.
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