生物
多元化(营销策略)
细胞
细胞生物学
计算生物学
细胞命运测定
转录因子
遗传学
基因
业务
营销
作者
Wei Wang,Xiang Niu,Tim Stuart,Estelle Jullian,William M. Mauck,Robert G. Kelly,Rahul Satija,Lionel Christiaen
标识
DOI:10.1038/s41556-019-0336-z
摘要
In vertebrates, multipotent progenitors located in the pharyngeal mesoderm form cardiomyocytes and branchiomeric head muscles, but the dynamic gene expression programmes and mechanisms underlying cardiopharyngeal multipotency and heart versus head muscle fate choices remain elusive. Here, we used single-cell genomics in the simple chordate model Ciona to reconstruct developmental trajectories forming first and second heart lineages and pharyngeal muscle precursors and characterize the molecular underpinnings of cardiopharyngeal fate choices. We show that FGF–MAPK signalling maintains multipotency and promotes the pharyngeal muscle fate, whereas signal termination permits the deployment of a pan-cardiac programme, shared by the first and second heart lineages, to define heart identity. In the second heart lineage, a Tbx1/10-Dach pathway actively suppresses the first heart lineage programme, conditioning later cell diversity in the beating heart. Finally, cross-species comparisons between Ciona and the mouse evoke the deep evolutionary origins of cardiopharyngeal networks in chordates. Wang et al. map early cardiopharyngeal development in the chordate model Ciona and show that FGF–MAPK signalling maintains multipotency and promotes pharyngeal muscle fate, whereas Tbx1/10-Dach specify second heart lineage identity.
科研通智能强力驱动
Strongly Powered by AbleSci AI