人类白细胞抗原
免疫沉淀
表位
肽
串联质谱法
抗原
HLA-B
HLA-A
化学
人口
生物
计算生物学
分子生物学
质谱法
抗体
生物化学
免疫学
色谱法
医学
环境卫生
作者
Amir Khan,Jiyon Shin,Min-Kyung So,Jung-Hyun Na,Sune Justesen,Adnan Ahmad Ansari,Byoung Chul Ko,Sung-Min Ahn
出处
期刊:Proteomics
[Wiley]
日期:2021-10-05
卷期号:: 2100171-2100171
标识
DOI:10.1002/pmic.202100171
摘要
Human leukocyte antigen (HLA) class I has more than 18,000 alleles, each of which binds to a set of unique peptides from the cellular degradome. Deciphering the interaction between antigenic peptides and HLA proteins is crucial for understanding immune responses in autoimmune diseases and cancer. In this study, we aimed to characterize the peptidome that binds to HLA-A*33:03, which is one of the most prevalent HLA-A alleles in the Northeast Asian population, but poorly studied. For this purpose, we analyzed the HLA-A*33:03 monoallelic B cell line using immunoprecipitation of HLA-A and peptide complexes, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this study, we identified 5731 unique peptides that were associated with HLA A*33:03, and experimentally validated the affinity of 40 peptides for HLA-A*33:03 and their stability in HLA A*33:03-peptides complexes. To our knowledge, this study represents the largest dataset of peptides associated with HLA-A*33:03. Also, this is the first study in which HLA A*33:03-associated peptides were experimentally validated.
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