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小RNA
核酸酶
复式(建筑)
G-四倍体
DNA
核糖核酸
计算生物学
生物
串联
底漆(化妆品)
分子生物学
化学
遗传学
聚合酶
基因
材料科学
复合材料
有机化学
作者
Liqi Liu,Fei Yin,Yu Lu,Xi-luan Yan,Chao‐Ming Wu,Xia Li,Chen-Zhong Li
标识
DOI:10.1016/j.nano.2020.102339
摘要
MicroRNA (miRNA) has emerged as a promising genetic marker for cancer diagnosis and therapy because its expression level is closely related to the progression of malignant diseases. Herein, a label-free and selective fluorescence platform was proposed for miRNA based on light-up "G-quadruplex nanostring" via duplex-specific nuclease (DSN) mediated tandem rolling circle amplification (RCA). First, a long DNA generated from upstream RCA was designed with the antisense sequences for miR-21 and downstream RCA primer. Upon recognizing miR-21, the resulting DNA-RNA permitted DSN digestion and triggered downstream two-way RCA, and generation of abundant "G-quadruplex nanostring" binding with ZnPPIX for label-free fluorescent responses. In our strategy, the strong preference of DSN for perfectly matched DNA/RNA ensures its excellent selectivity. The developed method generated wide linear response with LOD of 1.019 fM. Additionally, the miR-21 levels in cell extracts have been evaluated, revealing the utility of this tool for biomedical research and clinical diagnosis.
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