The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage

No AccessJournal of UrologyAdult Urology1 Jan 2021The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stageis corrected byErratum: Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage Bianca Oresta, Daniele Braga, Massimo Lazzeri, Nicola Frego, Alberto Saita, Cristina Faccani, Vittorio Fasulo, Piergiuseppe Colombo, Giorgio Guazzoni, Rodolfo Hurle, and Maria Rescigno Bianca OrestaBianca Oresta Humanitas Clinical and Research Center, Rozzano, Milan, Italy Recipient of a FIRC-AIRC fellowship. More articles by this author , Daniele BragaDaniele Braga Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Massimo LazzeriMassimo Lazzeri Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Nicola FregoNicola Frego Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Alberto SaitaAlberto Saita Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Cristina FaccaniCristina Faccani Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Vittorio FasuloVittorio Fasulo Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Piergiuseppe ColomboPiergiuseppe Colombo Department of Pathology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , Giorgio GuazzoniGiorgio Guazzoni Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Milan, Italy More articles by this author , Rodolfo HurleRodolfo Hurle Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author , and Maria RescignoMaria Rescigno †Correspondence: Department of Biomedical Sciences, Humanitas University, via Rita Levi Montalcini 4, 20090Pieve Emanuele, Milan , Italy telephone: +39 0282245431; E-mail Address: [email protected] Humanitas Clinical and Research Center, Rozzano, Milan, Italy Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Milan, Italy Supported by grants from the Italian Association for Cancer Research (AIRC; AIRC IG 22026 2018 and AIRC 5x1000 UniCanVax 22757), and the European Research council (ERC; HomeoGUT No. 615735). More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001336AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The dogma that urine is sterile has been overturned and dysbiosis of the urinary microbiome has been linked to many urological disorders. We tested the hypothesis that the urinary microbial composition may be different between men with or without bladder cancer in catheter collected urines, bladder washouts and midstream voided urines, and may be dependent on tumor staging. Materials and Methods: Liquid samples were collected from male patients with bladder cancer, and sex and age matched nonneoplastic controls. Total DNA was extracted and processed for 16S rRNA gene sequencing. Bioinformatic analysis for microbial classification was performed to assess diversity and variations. Results: The urinary microbiome associated with catheter collected urine samples of patients with bladder cancer was characterized by a significantly increased abundance of Veillonella (p=0.04) and Corynebacterium (p=0.03), and decreased Ruminococcus (p=0.03) compared to controls, with differences exacerbating with disease progression. Compared to catheterized urines, bladder cancer washouts showed the specific increase of some taxa, like Burkholderiaceae (p=0.014), whereas midstream urines were enriched in Streptococcus (p <0.0001), Enterococcus (p <0.0001), Corynebacterium (p=0.038) and Fusobacterium (p <0.0001). Conclusions: The bladder is colonized by endogenous bacteria and microbial modifications characterize the microbiome of patients with bladder cancer. Different microbial compositions can be characterized by changing sampling strategy. These results pave the way for exploring new diagnostic and therapeutic options based on the manipulation of the bacterial community. 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Google Scholar No direct or indirect commercial, personal, academic, political, religious or ethical incentive is associated with publishing this article. © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsRelated articlesJournal of Urology9 Feb 2021Erratum: Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage Volume 205Issue 1January 2021Page: 86-93Supplementary Materials Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.Keywordsmicrobiotatumor microenvironmenturinary bladder neoplasmsAcknowledgmentsWe thank the patients who participated in the clinical trial; the trial doctors, nurses and data manager, Nadia Lo Iacono, assisted with sample collection. Chiara Pozzi provided technical support and critical discussion.MetricsAuthor Information Bianca Oresta Humanitas Clinical and Research Center, Rozzano, Milan, Italy Recipient of a FIRC-AIRC fellowship. More articles by this author Daniele Braga Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Massimo Lazzeri Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Nicola Frego Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Alberto Saita Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Cristina Faccani Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Vittorio Fasulo Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Piergiuseppe Colombo Department of Pathology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Giorgio Guazzoni Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Milan, Italy More articles by this author Rodolfo Hurle Department of Urology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy More articles by this author Maria Rescigno Humanitas Clinical and Research Center, Rozzano, Milan, Italy Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, Milan, Italy †Correspondence: Department of Biomedical Sciences, Humanitas University, via Rita Levi Montalcini 4, 20090Pieve Emanuele, Milan , Italy telephone: +39 0282245431; E-mail Address: [email protected] Supported by grants from the Italian Association for Cancer Research (AIRC; AIRC IG 22026 2018 and AIRC 5x1000 UniCanVax 22757), and the European Research council (ERC; HomeoGUT No. 615735). 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