去细胞化
细胞外基质
再生医学
细胞生物学
干细胞
细胞分化
诱导多能干细胞
组织工程
生物
胚胎干细胞
遗传学
基因
作者
Saik Kia Goh,Willi Halfter,Thomas Richardson,Suzanne Bertera,Vimal Vaidya,Joe Candiello,Mahalia Bradford,Ipsita Banerjee
出处
期刊:Biofabrication
[IOP Publishing]
日期:2020-11-10
卷期号:13 (1): 015015-015015
被引量:5
标识
DOI:10.1088/1758-5090/abc05f
摘要
Pluripotent stem cells are promising source of cells for tissue engineering, regenerative medicine and drug discovery applications. The process of stem cell differentiation is regulated by multi-parametric cues from the surrounding microenvironment, one of the critical one being cell interaction with extracellular matrix (ECM). The ECM is a complex tissue-specific structure which is an important physiological regulator of stem cell function and fate. Recapitulating this native ECM microenvironment niche is best facilitated by decellularized tissue/organ derived ECM, which can faithfully reproduce the physiological environment with high fidelity toin vivocondition and promote tissue-specific cellular development and maturation. Recognizing the need for organ specific ECM in a 3D culture environment in driving phenotypic differentiation and maturation of hPSCs, we fabricated an ECM array platform using native-mimicry ECM from decellularized organs (namely pancreas, liver and heart), which allows cell-ECM interactions in both 2D and 3D configuration. The ECM array was integrated with rapid quantitative imaging for a systematic investigation of matrix protein profiles and sensitive measurement of cell-ECM interaction during hPSC differentiation. We tested our platform by elucidating the role of the three different organ-specific ECM in supporting induced pancreatic differentiation of hPSCs. While the focus of this report is on pancreatic differentiation, the developed platform is versatile to be applied to characterize any lineage specific differentiation.
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