PI3K/AKT/mTOR通路
癌症研究
蛋白激酶B
细胞凋亡
乳腺癌
活力测定
信号转导
癌症
激酶
转移
细胞生长
自噬
流式细胞术
生物
化学
医学
细胞生物学
免疫学
内科学
生物化学
作者
Xiaohu Li,Xin Zhang,Inam Ullah Khan,Nina Ni Guo,Bing Wang,Yuxin Guo,Bufan Xiao,Yueshan Zhang,Yi‐Min Chu,Jun Song Chen,Fang Guo
摘要
Abstract Background: Breast cancer is the main lethal disease among females. The combination of lobaplatin and microwave hyperthermia plays a crucial role in several kinds of cancer in the clinic, but its possible mechanism in breast cancer has remained indistinct. Methods: Mouse models were used to detect breast cancer progression. Cell growth was explored with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphonyl)-2H-tetrazolium) and colony formation assays. Cell migration and invasion were investigated with a transwell assay. Cell apoptosis was probed with flow cytometry. The expression of apoptosis-associated proteins was examined with Western blots. Result: Combination treatment decreased breast cancer cell viability, colony formation, cell invasion and metastasis. In addition, the treatment-induced breast cancer cell apoptosis and autophagy, activated the c-Jun N-terminal kinase (JNK) signaling pathway, suppressed the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway, and down-regulated IAP and Bcl-2 family protein expression. Conclusion: These results indicate that lobaplatin is an effective breast cancer anti-tumor agent. Microwave hyperthermia was a useful adjunctive treatment. Combination treatment was more efficient than any single therapy. The possible mechanism for this effect was mainly associated with activation of the JNK signaling pathway, inactivation of the AKT/mTOR signaling pathway and down-regulation of the Bcl-2 and IAP families.
科研通智能强力驱动
Strongly Powered by AbleSci AI