头孢吡肟
生物膜
铜绿假单胞菌
微生物学
最小抑制浓度
体外
拉伤
基因
生物
实时聚合酶链反应
基因表达
细菌
化学
分子生物学
抗菌剂
生物化学
头孢他啶
遗传学
解剖
作者
Soheir A. A. Hagras,Alaa El-Dien M.S. Hosny,Omneya M. Helmy,Mounir M. Salem‐Bekhit,Faiyaz Shakeel,Hala A. Farrag
出处
期刊:Canadian Journal of Microbiology
[Canadian Science Publishing]
日期:2021-12-01
卷期号:67 (12): 894-901
被引量:4
标识
DOI:10.1139/cjm-2021-0229
摘要
This study investigated the effect of cefepime at sub-minimum inhibitory concentrations (sub-MICs) on in vitro biofilm formation (BF) by clinical isolates of Pseudomonas aeruginosa. The effect of cefepime at sub-MIC levels (½-1/256 MIC) on in vitro BF by six clinical isolates of P. aeruginosa was phenotypically assessed following 24 and 48 h of challenge using the tissue culture plate (TCP) assay. Quantitative real-time polymeric chain reaction (qRT-PCR) was employed to observe the change in expression of three biofilm-related genes, namely, a protease-encoding gene (lasA), fimbrial protein-encoding gene (cupA1), and alginate-encoding gene (algC), in a weak biofilm-producing strain of P. aeruginosa following 24 and 48 h of challenge with sub-MICs of cefepime. The BF morphology in response to cefepime was imaged using scanning electron microscopy (SEM). The TCP assay showed strain-, time-, and concentration-dependent changes in in vitro BF in P. aeruginosa following challenge with sub-MICs of cefepime, with a profound increase in strains with inherently no or weak biofilm-producing ability. RT-PCR revealed time-dependent upregulation in the expression of the investigated genes following challenge with ½ and ¼ MIC levels, as confirmed by SEM. Cefepime at sub-MICs could upregulate the expression of BF-related genes and enhance BF by P. aeruginosa clinical isolates.
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