明胶
脚手架
体内
材料科学
生物医学工程
伤口愈合
再生(生物学)
硅酮
组织工程
体外
细胞外基质
离体
生物相容性
化学
去细胞化
生物材料
生物物理学
真皮
基质(化学分析)
外科
细胞生物学
医学
复合材料
生物
生物化学
生物技术
作者
Rostislav V. Shevchenko,Marc Eeman,Behzad Rowshanravan,Iain Allan,Irina N. Savina,Matthew Illsley,Michel Salmon,Stuart L. James,Sergey V. Mikhalovsky,Stuart James
标识
DOI:10.1016/j.actbio.2014.03.027
摘要
A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131 ± 17 μm at one surface decreasing to 30 ± 8 μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28 day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI