Evaluation of the chelating effect of chitosan solubilized in different acids

Aim: This study aimed to compare, through dentin microhardness and colorimetric analysis, the chelating effect of 0.2% chitosan solubilized in different acids.
Materials and Methods: The second and third cuts of the cervical region of maxillary central incisors were divided into four quadrants, resulting in eight specimens, which were treated with 50 μL of solution for 5 min according to their group (n = 10): GI – 0.2% chitosan solubilized in 1% acetic acid; GII – 0.2% chitosan solubilized in 3.3% citric acid; GIII – 0.2% chitosan solubilized in 0.00145% hydrochloric acid; and GIV – 0.2% chitosan solubilized in 0.00112% nitric acid. A control was made from the chelating properties of the following acids: GV – 3.3% citric acid, GVI – 0.00145% hydrochloric acid, GVII – 0.00112% nitric acid, and GVIII – control (distilled water). Afterward, they were subjected to the Knoop microhardness tester with a load of 10 g for 15 s, resulting in three indentations of the root canal toward the cement. The measurements obtained were subjected to the one-way ANOVA test followed by Tukey's test (α =0.05). Subsequently dispensing the chitosan solutions, the same were subjected to colorimetric analysis.
Results: Chitosan solubilized in acetic acid, followed by chitosan in citric acid, provided a greater reducing effect compared to the other groups. Similar results were observed in the colorimetric analysis.
Conclusion: It was concluded that the chelating ability of the chitosan solution solubilized in acetic acid is higher than solubilization in citric, hydrochloric, and nitric acids.