Preparation of pH-responsive magnetic nanocomposite hydrogels based on k-carrageenan/chitosan/silver nanoparticles: Antibacterial carrier for potential targeted anticancer drug delivery

This study reports the development of new pH-responsive drug delivery systems that are important for the treatment of cancer. The Mentha plant extract was obtained and then used for the biosynthesis of magnetic Ag bio nanoparticles (M-Ag bio-NPs). They were added in the formulation of hybrid hydrogel of k-carrageenan (k-Cr) and chitosan (CS) toward the synthesis of magnetic nanocomposite hydrogels. Their chemical structure and morphology were characterized by different analyses. Doxorubicin (DOX) was used as a model anticancer drug to study the targeted drug release behavior of the synthesized nanocomposite hydrogels (loading capacity: about 98 %). In vitro drug release studies showed that the release profile was noticeably controlled in a pH-dependent manner (higher drug release at pH 5). The antibacterial assessment confirmed the high antibacterial activity for the synthesized hydrogel against S. aureus (MIC values 39.06 μg/mL) and E. coli (MIC values > 19.53). In-vitro cytotoxicity results (MTT assay) demonstrated good biocompatibility (higher than 88 %) for the blank nanocomposite hydrogels, while DOX-loaded nanocomposite hydrogels showed high toxicity (about 22 % in the concentration of 20 μg/mL) against HeLa cells. The results showed that the present nanocomposite hydrogels can be suggested for potential application as an antibacterial and anticancer carrier.