纳米探针
吲哚青绿
光热治疗
体内
前哨淋巴结
转移
分子成像
化学
光动力疗法
荧光寿命成像显微镜
癌症研究
生物医学工程
材料科学
病理
医学
纳米技术
纳米颗粒
癌症
荧光
生物
内科学
有机化学
量子力学
乳腺癌
物理
生物技术
作者
Xueqian Wang,Geyang Dai,Guiying Jiang,Danya Zhang,Ling Wang,Wen Zhang,Chen Huang,Teng Cheng,Ying Zhou,Xiao Wei,Fēi Li,Ding Ma,Songwei Tan,Rui Wei,Xi Liu
标识
DOI:10.1186/s12951-023-01883-6
摘要
TMVP1 is a novel tumor targeting polypeptide screened by our laboratory with a core sequence of five amino acids LARGR. It specially binds to vascular endothelial growth factor receptor-3 (VEGFR-3), which is mainly expressed on neo-lymphatic vessels in sentinel lymph node (SLN) with tumor metastasis in adults. Here, we prepared a targeted nanoprobe using TMVP1-modified nanomaterials for tumor metastasis SLN imaging.In this study, TMVP1-modified polymer nanomaterials were loaded with the near-infrared (NIR) fluorescent dye, indocyanine green (ICG), to prepare a molecular imaging TMVP1-ICG nanoparticles (NPs) to identify tumor metastasis in SLN at molecular level. TMVP1-ICG-NPs were successfully prepared using the nano-precipitation method. The particle diameter, morphology, drug encapsulation efficiency, UV absorption spectrum, cytotoxicity, safety, and pharmacokinetic properties were determined. The TMVP1-ICG-NPs had a diameter of approximately 130 nm and an ICG loading rate of 70%. In vitro cell experiments and in vivo mouse experiments confirmed that TMVP1-ICG-NPs have good targeting ability to tumors in situ and to SLN with tumor metastasis by binding to VEGFR-3. Effective photothermal therapy (PTT) with TMVP1-ICG-NPs was confirmed in vitro and in vivo. As expected, TMVP1-ICG-NPs improved ICG blood stability, targeted tumor metastasis to SLN, and enhanced PTT/photodynamic (PDT) therapy, without obvious cytotoxicity, making it a promising theranostic nanomedicine.TMVP1-ICG-NPs identified SLN with tumor metastasis and were used to perform imaging-guided PTT, which makes it a promising strategy for providing real-time NIR fluorescence imaging and intraoperative PTT for patients with SLN metastasis.
科研通智能强力驱动
Strongly Powered by AbleSci AI