化学
乙醇沉淀
葡聚糖
多糖
平菇
单糖
水溶液
蘑菇
食用菌
香菇多糖
食品科学
作文(语言)
色谱法
产量(工程)
热稳定性
乙醇
生物化学
有机化学
语言学
哲学
材料科学
冶金
作者
Zaida Pérez-Bassart,Christine Bäuerl,María José Fabra,Antonio Martínez‐Abad,María Carmen Collado,Amparo López‐Rubio
标识
DOI:10.1016/j.ijbiomac.2023.127255
摘要
In this work, aqueous extracts from six different Pleurotus species were obtained and their yield, gross composition, β-glucan content, monosaccharide profile, thermal stability, molecular weight distribution, and FT-IR were analyzed before and after purification through ethanol precipitation of the carbohydrate-rich fractions. The bioactivity (anti-inflammatory and immunomodulatory activity) of the various fractions obtained was also analyzed in three different cell cultures and compared with a lentinan control. The trend observed after purification of the aqueous fractions was an increase in the concentration of polysaccharides (especially β-glucans), a decrease in ash, glucosamine and protein content and the elimination of low molecular weight (Mw) compounds, thus leaving in the purified samples high Mw populations with increased thermal stability. Interestingly, all these purified fractions displayed immunomodulatory capacity when tested in THP-1 macrophages and most of them also showed significant activity in HEK-hTLR4 cells, highlighting the bioactivity observed for Pleurotus ostreatus (both the extracts obtained from the whole mushroom and from the types). This specific species was richer in heteropolysaccharides, having moderate β-glucan content and being enriched upon purification in a high Mw fraction with good thermal stability.
科研通智能强力驱动
Strongly Powered by AbleSci AI