Detection and quantification of nanoparticle-induced intracellular ROS in live cells by laser scanning confocal microscopy

• ROS elevation in HT-1080 by Ce-based nanoparticle treatment. • H 2 DCFDA-mediated detection of ROS by laser scanning confocal microscopy . • Writing of custom-built MATLAB software for image analysis of ROS fluorescence intensities. • Processing of images for quantification of fluorescence intensities. • Statistical analysis by ANOVA to confirm statistical significance.