Metagenomic Analysis of Brain Abscesses Identifies Specific Bacterial Associations

基因组 菌群(微生物学) 聚合酶链反应 核糖体RNA 核糖体DNA 脑脓肿 医学 16S核糖体RNA 微生物培养 微生物学 生物 脓肿 细菌 遗传学 系统发育学 基因
作者
Mouhamad Al Masalma,M. Lonjon,Hervé Richet,Henry Dufour,Pièrre-Hugues Roche,Michel Drancourt,Didier Raoult,Pierre-Édouard Fournier
出处
期刊:Clinical Infectious Diseases [Oxford University Press]
卷期号:54 (2): 202-210 被引量:116
标识
DOI:10.1093/cid/cir797
摘要

The bacterial flora involved in brain abscess is often complex. In a previous study, using a metagenomic approach based on 16S ribosomal DNA (rDNA) amplification, we demonstrated that the diversity of the microbial flora involved in these infections was underestimated.We performed a 16S rDNA-based metagenomic analysis of cerebral abscesses from patients diagnosed from 2006 through 2010. All bacteria present in brain abscess specimens were identified, in view of the clinical and epidemiological characteristics of the patients.Fifty-one patients were included in our study. By detecting polymicrobial infections in 19 patients, our strategy was significantly more discriminatory and enabled the identification of a greater number of bacterial taxa than did culture and conventional 16S rDNA polymerase chain reaction (PCR) and sequencing, respectively (P < 10(-2)). Data mining discriminated 2 distinct bacterial populations in brain abscess from dental and sinusal origin. In addition, of the 80 detected bacterial species, we identified 44 bacteria that had never been found in brain abscess specimens, including 22 uncultured bacteria. These uncultured agents mostly originated from the buccal or sinusal floras (P < 10(-2)) and were found in polymicrobial specimens (P < 10(-2)).Cloning and sequencing of PCR-amplified 16S rDNA is a highly valuable method to identify bacterial agents of brain abscesses.
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