First Report of Colletotrichum gloeosporioides Causing Anthracnose on Grapevine (Vitis vinifera) in Shaanxi Province, China

HomePlant DiseaseVol. 107, No. 7First Report of Colletotrichum gloeosporioides Causing Anthracnose on Grapevine (Vitis vinifera) in Shaanxi Province, China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Colletotrichum gloeosporioides Causing Anthracnose on Grapevine (Vitis vinifera) in Shaanxi Province, ChinaYanchun Fan, Fangyin Guo, Ronghui Wu, Ziqiu Chen, and Zhi LiYanchun FanCollege of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China, Fangyin GuoState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China, Ronghui WuState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China, Ziqiu ChenState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China, and Zhi Li†Corresponding author: Z. Li; E-mail Address: [email protected]https://orcid.org/0000-0003-4144-0099State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaAffiliationsAuthors and Affiliations Yanchun Fan1 Fangyin Guo2 Ronghui Wu2 Ziqiu Chen2 Zhi Li2 † 1College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China 2State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China Published Online:6 Jul 2023https://doi.org/10.1094/PDIS-10-22-2385-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleGrape anthracnose caused by Colletotrichum spp. is an economically important disease in most vineyards during rainy and humid weather. In June 2021, widespread anthracnose was observed on leaves of Vitis vinifera 'Red Globe' in Yangling, Shaanxi Province, China (34.28°N, 108.07°E). Diseased plants exhibited circular brown necrotic lesions with yellow borders on young leaves. Approximately 80% of the 75 grape plants showed disease symptoms that were consistent with symptoms previously reported for anthracnose on grape caused by C. acutatum, C. gloeosporioides, and C. viniferum (Hong et al. 2008; Oo and Oh 2017). To isolate the pathogen, 10 diseased leaves were collected. Small pieces (0.5 × 0.5 mm) of symptomatic tissues were surface sterilized with 75% alcohol for 30 s, soaked in 4% NaClO for 2 min, and then washed twice with sterile distilled water. The pathogen was isolated and cultured on potato dextrose agar (PDA) at 25°C with a 12-h light/dark photoperiod. Hyphal tips of the isolate were transferred to fresh PDA after 1 day. Acervuli were formed on PDA after 15 days. The colonies were single-spored to obtain pure cultures. Pure cultures on PDA appeared white to grey, and the reverse side of the colonies was olive gray or yellowish white. Conidia were single-celled, hyaline, straight, and cylindrical with rounded ends and measured 12.50 to 17.78 μm (length) (mean = 15.19 μm, n = 50) × 3.66 to 5.82 μm (width) (mean = 4.48 μm, n = 50). The morphological characteristics matched the previous descriptions of C. gloeosporioides by Sawant et al. (2012). For molecular identification, genomic DNA was extracted from the isolate YLRG13, and actin (ACT), internal transcribed spacer (ITS), and beta-tubulin (TUB2) were amplified using the primer pairs ACT-512F/ACT-783R (Carbone and Kohn 1999), ITS1F/ITS4 (White et al. 1990), and T1/T2 (O'Donnell and Cigelnik 1997), respectively. ACT (OQ031183 and MZ686707), ITS (OP999326 and MZ669974), and TUB2 (OQ031184 and MZ686708) nucleotide sequences of the isolates YLRG5 and YLRG13 were deposited in GenBank. BLASTn showed 99 to 100% similarity with C. gloeosporioides. Neighbor-joining trees based on the three genes were constructed using PAUP version 4.0b10. The results showed the isolates YLRG5 and YLRG13 from V. vinifera were closely related to C. gloeosporioides with high bootstrap values. To test the pathogenicity of the isolate YLRG13, 10 μl of conidial suspension (4 × 108 conidia/ml) was inoculated on the surfaces of five wounded detached leaves of 6-year-old V. vinifera 'Red Globe'. Sterile distilled water was used as a control. Inoculated plants were placed in a humid chamber at 25°C. Conidial germination was observed at 24 h postinoculation. Four days after inoculation, typical anthracnose symptoms developed on inoculated grape leaves. No symptoms were observed on the controls. The fungus reisolated from the symptomatic grape leaves showed the same morphological characteristics of the inoculated isolate, fulfilling Koch's postulates. To our knowledge, this is the first report of grape anthracnose caused by C. gloeosporioides in the northwestern region of China. C. gloeosporioides was reported causing anthracnose on a wide variety of horticultural crops, such as almond, avocado, apple, banana, cashew, citrus, cucumber, grape, guava, mango, onion, papaya, passion fruit, pepper, strawberry, tomato, and watermelon (Sharma and Kulshrestha 2015). This study suggests potential cross-infection between grape and other horticultural crops.The author(s) declare no conflict of interest.References:Carbone, I., and Kohn, L. M. 1999. Mycologia 91:554. https://doi.org/10.2307/3761358 Crossref, Google ScholarHong, S. K., et al. 2008. Plant Pathol. J. 24:270. Crossref, Google ScholarO'Donnell, K., and Cigelnik, E. 1997. Mol. Phylogenet. Evol. 7:103. https://doi.org/10.1006/mpev.1996.0376 Crossref, ISI, Google ScholarOo, M. M., and Oh, S.-K. 2017. Mycobiology 45:421. https://doi.org/10.5941/MYCO.2017.45.4.421 Crossref, ISI, Google ScholarSawant, I. S., et al. 2012. Australas. Plant Pathol. 41:496. https://doi.org/10.1007/s13313-012-0143-5 Crossref, Google ScholarSharma, M., and Kulshrestha, S. 2015. Biosci. Biotechnol. Res. Asia 12:1234. Crossref, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarFunding: This work was supported by the National Natural Science Foundation of China (32072534).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 107, No. 7 July 2023SubscribeISSN:0191-2917e-ISSN:1943-7692 Download Metrics Article History Issue Date: 26 Jul 2023Published: 6 Jul 2023First Look: 23 Jan 2023Accepted: 18 Jan 2023 Page: 2249 Information© 2023 The American Phytopathological SocietyFundingNational Natural Science Foundation of ChinaGrant/Award Number: 32072534KeywordsanthracnoseColletotrichum gloeosporioidesfungal diseaseVitis viniferaThe author(s) declare no conflict of interest.PDF download