Wnt信号通路
上皮-间质转换
转移
癌症研究
结直肠癌
连环素
激活剂(遗传学)
信号转导
生物
医学
化学
癌症
内科学
细胞生物学
受体
作者
Ziwei Wang,Ya-Sai Sun,Mengying Wu,Liangfu Zhou,Zheng Yu,Ting Ren,Meijiao Li,Wen Zhao
出处
期刊:Foods
[Multidisciplinary Digital Publishing Institute]
日期:2024-04-12
卷期号:13 (8): 1171-1171
被引量:1
标识
DOI:10.3390/foods13081171
摘要
Colorectal carcinoma (CRC) is a major global health concern, with cancer metastasis being the main cause of patient mortality, and current CRC treatments are challenged by drug resistance. Although natural compounds, especially in foods like hawthorn proanthocyanidin extract (HPOE), have good anticancer activity, their effects on CRC metastasis remain unknown. Therefore, our objective was to investigate the impact and potential mechanisms of HPOE on the movement and infiltration of cells in the HCT116 CRC cells. Firstly, scratch-healing experiments confirmed the anti-migratory and anti-invasive capabilities of HPOE. Then, network pharmacology identified 16 possible targets, including MMP-9. Subsequently, RT-qPCR and Western blotting experiments confirmed that HPOE downregulated epithelial-mesenchymal transition-related factors (N-cadherin and MMP-9) and inhibited Wnt/β-catenin pathway activation. Finally, these results were experimentally validated using the Wnt pathway activator Licl and inhibitor XAV939. It was confirmed that HPOE had a certain inhibitory effect on the activation of the Wnt signaling pathway caused by the activator Licl and could enhance the inhibitory effect of the inhibitor XAV939. Our findings provide a basis for developing functional foods or dietary supplements, especially positioning HPOE as a functional food raw material for adjuvant treatment of CRC, given its ability to inhibit metastasis through the Wnt/β-catenin pathway.
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