生物
克隆(Java方法)
系统发育树
B细胞
系统发育学
B细胞受体
细胞
单细胞分析
遗传学
核糖核酸
转录组
突变
免疫系统
基因
计算生物学
进化生物学
抗体
基因表达
作者
Kenneth B. Hoehn,Steven H. Kleinstein
标识
DOI:10.1016/j.it.2023.11.004
摘要
The widespread availability of single-cell RNA sequencing (scRNA-seq) has led to the development of new methods for understanding immune responses. Single-cell transcriptome data can now be paired with B cell receptor (BCR) sequences. However, RNA from BCRs cannot be analyzed like most other genes because BCRs are genetically diverse within individuals. In humans, BCRs are shaped through recombination followed by mutation and selection for antigen binding. As these processes co-occur with cell division, B cells can be studied using phylogenetic trees representing the mutations within a clone. B cell trees can link experimental timepoints, tissues, or cellular subtypes. Here, we review the current state and potential of how B cell phylogenetics can be combined with single-cell data to understand immune responses.
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